M7443
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 | Immunoblotting Jurkat cell nuclear extracts were separated on SDS-PAGE and blotted with Monoclonal Anti-MECP2 antibodies. (Lane 1) clone Men-8 (4 μg/mL) (Cat. No. M7443); (Lane 2) clone Mec-168 (0.5 μg/mL) (Cat. No. M6818). |
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M7443
Sigma
Monoclonal Anti-MeCP2 antibody produced in mouse
clone Men-8, purified immunoglobulin, buffered aqueous solution
| MDL number: | MFCD02686372 |
Description
| Immunogen | synthetic peptide corresponding to the N-terminus (amino acids 15-30) of human MeCP2. |
| Physical form | Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | Men-8 |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~75 kDa |
| species reactivity | rat, human, monkey, mouse |
| application(s) | immunoblotting: 1-2 μg/test using nuclear extract of cultured human acute T cell leukemia Jurkat cells or MCF7 cells |
| immunocytochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| isotype | IgG1 |
| Gene Information | human ... MECP2(4204) |
| rat ... Mecp2(29386) | |
| mouse ... Mecp2(17257) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
